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. 2019 Feb 13;4(1):e00586-18. doi: 10.1128/mSphere.00586-18

FIG 4.

FIG 4

Differential effects of peptide treatment on in vivo- and in vitro-derived MCMV. Peptide at a 50 µM concentration was used to inhibit (A) tissue culture-derived (TCV) or (B) salivary gland-derived (SGV) MCMV. (C) Data from panels A and B to compare the efficacy of p5 + 14(coil) inhibition of TCV and SGV. (D) MEF 10.1 cells were treated with 50 mM sodium chlorate to remove 2-O- and 6-O-linked sulfations. Treated cells were infected with ∼100 PFU of TCV or SGV. Either TCV (E) or SGV (F) was incubated with various concentrations of heparin and used to infect MEF 10.1 cells (∼100 PFU/well). Data were normalized to untreated (PBS) controls. Bars represent the average ± SD from 2 experiments with 3 replicates per experiment. Statistical significance was determined by one-way ANOVA with a Tukey’s multiple comparison of means. ns, not significant; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001.