FIG 7.
Peptide inhibition of MCMV cell-to-cell spread. (A) MEF 10.1 cells were treated with PBS, p5(coil), or p5 + 14(coil) at 100 μM 1 h prior to incubation with 1 × 105 MCMV-infected peritoneal exudate cells. (B) MEF10.1 cells were treated with p5 + 14(coil) at 50 μM 1 h prior to incubation with either cell-free MCMV or 300 MEF 10.1 cells infected with MCMV. (C) MEF 10.1 cells were treated with 12 U/μl of heparinase for 1 h, 50 mM sodium chlorate overnight, or 40 µg/ml of heparin and then infected with cell-associated MCMV. Bars represent the average from 2 experiments with 3 or 4 replicates per experiment. Statistical significance was determined by an ordinary one-way ANOVA with a Tukey’s multiple comparison of means or an unpaired t test. ns, not significant; *, P ≤ 0.05; **, P ≤ 0.01; ****, P ≤ 0.0001.