. 2019 Feb 13;4(1):e00637-18. doi: 10.1128/mSphere.00637-18

TABLE 2.

Overview of IFN-α2 mutants used in this study

Mutant	Description of mutation(s)	Binding affinity for^a :		Anti-HIV activity (P value)^b	Reference(s)^c
Mutant	Description of mutation(s)	IFNAR1	IFNAR2	Anti-HIV activity (P value)^b	Reference(s)^c
IFN-α2-145G	A145G	1	−33×	−24.94 (<0.0001)	33
IFN-α2-26A	L26A	1	−5×	−2.364 (0.9789)	34
IFN-α2-α8T	C-terminal tail replaced with that of IFN-α8	1	+20×	16.46 (0.0015)	34
YNS	H57Y E58N Q61S	+60×	1	19.68 (0.0002)	35
YNS-α8T	YNS + C-terminal tail replaced with that of IFN-α8	+60×	+20×	15.55 (0.0027)	36
YNS-148A	YNS + M148A	+60×	−60×	10.5 (0.0608)	1, 24, 25

Binding affinity compared to that of IFN-α2.

Mean difference in V_res results compared to the result for IFN-α2 in CD4⁺ T cells (see Fig. 4B). Significance testing was done using RM one-way ANOVAs, comparing all mutants to IFN-α2.

The references cited in the last column are the papers that assessed the mutants’ binding affinities for IFNAR1 and IFNAR2.