TABLE 4.

Group A Streptococcus candidate vaccine antigen genomic screen^c

Gene/antigen	Gene identifiera	Function	M75 611024	M12 611025	M1T1 5448
		M-protein, N terminal (30-valent vaccine)	✓	✓	✓
		M-protein, C terminal (J8.0)		✓	✓
		M-protein, C terminal (StreptInCor T-cell epitope)
		M-protein, C terminal (StreptInCor B-cell epitope)
		M-protein, C terminal (StreptInCor common epitope)		✓
adi	MGAS5005_spy1275	Arginine deaminase	✓	✓	✓
fbaA	MGAS5005_spy1714	Fibronectin-binding protein A
fbp54	AAA57236	Fibronectin-binding protein 54	✓	✓	✓
oppA	M5005_spy0249	Oligopeptide-binding protein	✓	✓	✓
GAC	MGAS5005b	Group A carbohydrate	✓	✓	✓
pulA	SF370_spy1972	Putative pullulanase	✓	✓	✓
r28	AF091393	Rib-like cell wall protein
scpA	MGAS5005_spy1715	C5a peptidase	✓	✓	✓
sfbI	X67947	Streptococcal fibronectin binding protein I
sfbII-sof	X83303	Serum opacity factor	✓
shr	SPY1530	Streptococcal hemoprotein receptor	✓	✓	✓
sib35	AB254157	Streptococcal immunoglobulin-binding protein 35	✓	✓	✓
slo	M5005_spy0124	Streptolysin O	✓	✓	✓
spa	MGAS8232_spyM18_2046	Streptococcal protective antigen
speA	X03929	Streptococcal pyrogenic exotoxin A			✓
speB	M5005_spy1735	Cysteine protease	✓	✓	✓
speC	SF370_spy0711	Streptococcal pyrogenic exotoxin C
spy0651	MGAS5005_spy0651	Cell surface protein	✓	✓	✓
spy0762	MGAS5005_spy0762	Hypothetical membrane associated protein	✓	✓	✓
spy0942	MGAS5005_spy0942	Nucleoside-binding protein	✓	✓	✓
spyAD	MGAS5005_spy0229	Adhesin and division protein	✓	✓	✓
spyCEP	MGAS5005_spy0341	Interleukin-8 serine protease	✓	✓	✓
sse	SF370_spy1407	Serine esterase		✓	✓
tee	MGAS5005_spy0109	T antigen	✓	✓	✓
tif	SF370_spy1612	Trigger factor	✓	✓	✓

^aNucleotide gene sequences derived from completely sequenced genomes or listed GenBank identifiers. Accession numbers for genome sequences include MGAS5005 (CP000017), SF370 (AE004092), and MGAS8232 (AE009949).

^bGAC operon (∼14.2 kb) refers to MGAS5005 genome coordinates 604873 to 619151.

^cBLAST analyses at a homology level of 80% for protein antigens and 100% for peptide-derived sequences.