Figure 2.

Effects of EP on C57BL/6 mice DC. BMDC were obtained from C57BL/6 mice. They were treated with EP (3.1 mM) for 24 h simultaneously with LPS (“matEP”) or at days 3 and 6 of the propagation (“difEP”) or they were untreated with EP (mDC), as schematized (A). Viability (B) was determined by trypan blue exclusion assay. Cytokines were determined in cell culture supernatants by ELISA (C–F). Proportion of cells expressing the listed antigens was determined by cytofluorimetry (G–J). Data are presented as mean + SD from at least three independent experiments. Data in (J) are representative plots. ^*p < 0.05, EP vs. mDC, one way ANOVA followed by Tukey's multiple comparisons test.