Figure 1.

Key features of RTgutGC cells grown on conventional culture plates or transwell membranes. (A) Viability of RTgutGC cells in 24-well-culture plates with 1.5 × 10⁵ cells/mL (78,947 cells per cm²) exposed to mucosal saline for 12 h. (B) TEER of RTgutGC cells grown up to 4 weeks in 24-well-culture plates with membrane inserts at initial density of 8 × 10⁴ cells/mL (71,429 cells per cm²). (C) Fluorescent levels in basolateral media after fluorescent albumin exposure into apical chamber in 24-well-transwell membrane plates with or without RTgutGC cells. (D) Confocal fluorescence microscopy images of the tight junction protein claudin 3 (red) and the nuclei (blue) in RTgutGC cells grown on conventional culture plates. (E,F) Uptake of albumin (E) and zymosan (F) during the 3 h exposure time with cells cultured in conventional 6-well plates. For both panels (E,F), X axis shows the fluorescence signal from albumin or zymosan in cells. Y axis shows the percentage of albumin/zymosan positive cells out of total live cell population. Data represent mean ± SEM of two independent experiments with 3–4 technical replicates each (wells or inserts). Scale bar = 100 μm.