Fig. 1. Schematic representation of the inorganic cofactor constituting the active site of [FeFe]-hydrogenase and the model complexes employed in this study. (A): The complete H-cluster; (B): the [2Fe] subsite model [Fe2(adt)(CO)4(CN)2]2–, 1; (C): a truncated structure of P. aerogenes ferredoxin I, the -CIACGAC- core motif on which the FdM maquette is based is highlighted in yellow. Heteroatom colour coding: Fe = orange; S = yellow; N = blue and O = red. A and C generated from Protein Data Bank entries ; 3C8Y (selected amino acids removed to provide an unobstructed view of the cofactor) and ; 1DUR respectively.
