Fig. 1 .

Everolimus inhibits the growth of pLGG lines. (A) Western blot of phospho-S6, total S6, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in Res186, Res259, BT66, and JHH-NF1-PA1. Cells were incubated with 0, 0.1, 1, or 10 nM of everolimus for 6 hours. (B) MTS assay of cellular proliferation after cells were treated with 0.1–10 nM everolimus. Each condition was performed in triplicate. (C) Quantification of BrdU. Bars represent ±SD. Experiment was performed in 3 distinct biological replicates. Bars represent ±SD; *P < 0.05, ***P < 0.001, ****P < 0.001 against 0 nM by 2-tailed t-test.