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. 2019 Feb 13;16:8. doi: 10.1186/s12989-019-0291-7

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Quantification of chromosomal damage and cell viability of 16HBE14o- cells following dSPION exposure. a Mono-cultured 16HBE14o- cells treated with γ-Fe2O3 b 16HBE14o- cells treated with γ-Fe2O3 DTHP-1macrophage conditioned media c 16HBE14o- cells pre-treated with NAC and exposed to γ-Fe2O3 DTHP-1 macrophage conditioned media. d Mono-cultured 16HBE14o- cells treated with Fe3O4 e 16HBE14o- cells treated with Fe3O4 DTHP-1 macrophage conditioned media f 16HBE14o- cells pre-treated with NAC and exposed to Fe3O4 DTHP-1 macrophage conditioned media, *p < 0.05 when compared to negative control (0 μg/ml). For all CBMN assays MMC (0.01 mg/ml) was used as a positive control – (micronuclei fold increase 2.5–2.8%) (n = 3)