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. 2018 Jan 4;13(3):636–646. doi: 10.1021/acschembio.7b01039

Figure 2.

Figure 2

Hsp70 inhibits tau elongation. (a) Seeded aggregation of K18 ΔK280 tau in the presence of Hsp70. The increase of relative aggregate mass upon the addition of free monomers (10 μM) to existing seeds (10 μM) was measured over time by ThT fluorescence and normalized. Black line, tau only; gray line, + 0.5 μM Hsp70 (1:20 Hsp70/tau); red line, + 2 μM Hsp70 (1:5 Hsp70/tau); blue line, + 10 μM Hsp70 (1:1 Hsp70/tau). N = 3, error bars were omitted for the sake of clarity. Inset: Representative linear fits of the initial aggregation rate to derive the elongation rate of seeds as a function of Hsp70 concentration. (b) Relative elongation rates (k+′) of tau seeds as a function of Hsp70 concentration. Error bars: st. dev. of experimental repeats. Statistical test: One-way ANOVA; **p ≤ 0.01, ****p ≤ 0.0001.