Figure 3.

CAF-EV promote a budding pattern of invasion. Pooled NOF- or CAF-EV were cultured with HSC-3 cells and let to invade within the 3D-myoma organotypic model for 14 days. (a) Representative images of the invaded cells stained with pan-cytokeratin (scale bars represent 400 µm). (b) Quantification of the invaded cells. CAF-EV induced HSC-3 cells to invade deeper and in smaller tumour cell islands compared to the NOF-EV treated and to the control cells (HSC-3 cells without EV). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.