Fig. 2.

Variability and efficiency tests for transcripts of interest using EvaGreen dPCR. A) Variability. Ten replicates were performed per concentration for each transcript (nine for ACTB 100 ag), and coefficient of variation calculated for each. B) Efficiency. Five dilution replicates were performed per concentration for each transcript (four for ACTB 10 fg, ACTB 1 fg, ACTB 100 ag, CEBPA 1 fg, PU.1 1 fg), and mean efficiency values calculated for each. More information can be found in Table 1 and values used for calculations are included in the Supplementary Information.