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. 2019 Jan 30;4(1):2331–2336. doi: 10.1021/acsomega.8b03571

Figure 1.

Figure 1

Met inhibits the LPS-induced inflammatory stress in RAW 264.7 macrophages. RAW 264.7 cells were pretreated with 10 mM Met for 12 h prior to stimulation with 100 μg/mL LPS for 3 h. The gene expression of (A) IL-6, (B) TNF-α, and (C) IFN-β was analyzed by RT-qPCR. (D–F) Effects of Met on LPS-stimulated IL-6, TNF-α, and IFN-β secretion from RAW 264.7 cells were analyzed by ELISA. Cells were cultured for 12 h with Met (10 mM) and then treated with LPS (100 ng/mL) for 12 h. Data represent the mean ± SD of three independent experiments, each performed in five samples. Comparisons among means used t-tests (*p < 0.05, **p < 0.01, ***p < 0.001).