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. 2019 Jan 30;4(1):2331–2336. doi: 10.1021/acsomega.8b03571

Figure 2.

Figure 2

Met inhibits the LPS-induced phosphorylation of mitogen-activated protein kinases (MAPK) in RAW 264.7 macrophages. RAW 264.7 cells were cultured for 12 h with Met (10 mM) and then treated with LPS (100 ng/mL) for 0, 5, 15, 30, and 60 min. Western blotting assays for phospho-ERK1/2 (p-ERK1/2), ERK1/2, p-JNK, JNK, p-p38, and p38 were performed. β-Actin was used as the loading control. Levels for target phosphorylated proteins were normalized to total proteins. All data shown are representative of three independent experiments.