Table 3.
Oligos used for spacer annealing and sequence verification.
| Oligo name | Sequence (5–3′)a |
|---|---|
| Forward | |
| sul2_gRNA.fwd | gaagCGCTGCGTTCTATCCGCAAT |
| OXA55_gRNA.fwd | gaagCTGGCCAACTGCTGATACAC |
| NmcR_gRNA.fwd | gaagTTGGGGCCGTTGAGCAGCAT |
| Reverse | |
| sul2_gRNA.rev | aaacATTGCGGATAGAACGCAGCG |
| OXA55_gRNA.rev | aaacGTGTATCAGCAGTTGGCCAG |
| NmcR_gRNA.rev | aaacATGCTGCTCAACGGCCCCAA |
| gRNA screen primer pair | |
| gRNA.screenF | CACTTAACGGCTGACATGGGAATTAGC |
| gRNA.screenR | CTGGCTTTCTACGTGTTCCGCTTCC |
a
Lowercase are sequences complementary to the flanking vector sequences generated by the BsaI cut.