Table 4.
Primers used for donor DNA construction.
| Primer name | Sequence (5–3′)a |
|---|---|
| L arm forward | |
| sul2_Larm.fwd | ccgtattaccgcctttgagtgagctcGGCTACTTGAATGAGCAAACTGGCAG |
| OXA-55_Larm.fwd | ccgtattaccgcctttgagtgagctcTATCTCAAGTCCAAAGGAACGCCG |
| NmcR_Larm.fwd | ccgtattaccgcctttgagtgagctcCGCGATAATATATCGCTGCTGAGTCG |
| L arm reverse | |
| sul2_Larm.rev | cggtttctttcagcgGATGAGCGATTTATTCATGGGGGCT |
| OXA-55_Larm.rev | aataaaaggggaatgagGTCTGGCTGGCACGGCATAG |
| NmcR_Larm.rev | cgttacccaggccCGTTTGGTCTGTCCGTTCCAGG |
| R arm forward | |
| sul2_Rarm.fwd | atgaataaatcgctcatcCGCTGAAAGAAACCGCAAGAATTCG |
| OXA-55_Rarm.fwd | gtgccagccagacCTCATTCCCCTTTTATTCCCGGCG |
| NmcR_Rarm.fwd | acggacagaccaaacgGGCCTGGGTAACGAACAGTTCTTC |
| R arm reverse | |
| sul2_Rarm.rev | attttgttatcattcccctagagctcAAGTCGGGATTGACGGCATTGC |
| OXA-55_Rarm.rev | attttgttatcattcccctagagctcATCTGCAACCGGCTCCAGTAAAG |
| NmcR_Rarm.rev | attttgttatcattcccctagagctcAAGCTGAACCTTTGATGGACTGACTG |
| donor DNA screen primer pair | |
| Donor.screenF | CACTTAACGGCTGACATGGGAATTAGC |
| Donor.screenR | CTGGCTTTCTACGTGTTCCGCTTCC |
a
Lowercase are sequences complementary to the flanking vector sequences generated by the BsaI cut.