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Primers used for target gene deletion PCR screen.
| Primer name | Sequence (5–3′) |
|---|---|
| Forward | |
| sul2_del_screen.fwd | GGCCATGAAGGCCGCTTATTGA |
| OXA-55_del_screen.fwd | AACTGGATGTTCAGTTTACCGATGCC |
| NmcR_del_screen.fwd | CTGAAGATGTGTCTGCGGTTCATGG |
| Reverse | |
| sul2_del_screen.rev | CCTAAAACTCTTCAATGCACGGGTCT |
| OXA-55_del_screen.rev | GGTCGAATCCGGTCAGCAGTATC |
| NmcR_del_screen.rev | AGACCTATCTGCTCTATTGCGATCGC |
