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. 2019 Feb 6;22:101118. doi: 10.1016/j.redox.2019.101118

Fig. 3.

Fig. 3

Impaired phagocytic response and migration of Cx3cr1-deficient microglia. (A) Microglia from Cx3cr1+/+ or Cx3cr1−/− mice were incubated with fluorescent microspheres in the absence or presence of 100 nM of CX3CL1 for 2 h. Phagocytic efficiency was calculated as a number of microspheres per cell. One-way ANOVA followed by Newman–Keuls test was used to assess differences among groups. Asterisks denote significant differences: *p < 0.05, **p < 0.01, ***p < 0.001 compared with the indicated groups. (B) Motility was determined by using CytoSelect 96-well cell migration assay from primary cultures of microglia from Cx3cr1+/+ and Cx3cr1−/− mice in accordance with the manufacturer's instructions, described in “Materials and Methods”. Positive control: 15% FBS; SFN (5 μM); CX3CL1 (100 nM) for 16 h. Values are mean±SEM (n = 3, performed two times).