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. 2019 Feb 6;22:101118. doi: 10.1016/j.redox.2019.101118

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. S1 — Astrocytes from Cx3cr1^-/-mice do not shown any alterations in the transcription factor NRF2 signalling. Primary cultures of astrocytes from Cx3cr1^+/+ and Cx3cr1^−/− mice were incubated with SFN (15 μM, 6 h). Quantitative real-time PCR determination of messenger RNA levels of NRF2-regulated genes coding Nfe2l2, Nqo1, Gclc and Osgin1, respectively, normalized by Actb (β-Actin) messenger RNA levels. Two-way ANOVA followed by Bonferroni post-test was used to assess significant differences among groups. Asterisks denote significant differences ***p < 0.001 respect to the basal Cx3cr1^+/+ group.