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. 2019 May;14(5):783–793. doi: 10.4103/1673-5374.249226

Figure 1.

Figure 1

Effect of SXT on cell viability and apoptosis-related protein expression in bEnd.3 cells subjected to OGD/R.

bEnd.3 cells subjected to 6-hour OGD followed by reperfusion for 18 hours were administered Shuxuetong injection at 1/32, 1/64, and 1/128 concentrations (diluted 32-, 64-, and 128-times). (A) Cell viability detected by Cell Counting Kit-8 assay was analyzed at 24 hours after OGD/R. (B) LDH leakage detected by fluorescence analysis was analyzed at 24 hours after OGD/R. (C, D) Cleaved caspase-3 and Bcl-2 protein expression detected by western blot assay was analyzed at 24 hours after OGD/R. Relative protein expression was expressed as optical density value relative to control group after normalizing to GAPDH optical density value. Data are expressed as the mean ± SD (mean from three independent experiments, one-way analysis of variance followed by the least significant difference test). #P < 0.05, vs. control group; *P < 0.05, vs. OGD/R group (n = 6). SXT: Shuxuetong injection; OGD/R: oxygen-glucose deprivation/reperfusion; LDH: lactate dehydrogenase GAPDH: glyceraldehyde-3-phosphate dehydrogenase.