Figure 4.

Effect of SXT on expression of inflammatory cytokines induced in bEnd.3 cells by OGD/R injury.

Cells subjected to 6-hour OGD followed by reperfusion for 18 hours were administered Shuxuetong injection at 1/32, 1/ 64, and 1/128 concentrations (diluted 32-, 64-, and 128-times). (A, B) ICAM-1 and VCAM-1 protein expression levels were detected by western blot assay. Relative protein expression was expressed as optical density value relative to the control group after normalizing to β-actin optical density value. (C–F) iNOS, TNF-α, IL-1β, and IL-6 mRNA expression levels were detected by real-time polymerase chain reaction. mRNA expression levels were determined relative to a blank control after normalizing to GAPDH using the 2^−ΔΔCT method. Data are expressed as the mean ± SD (mean from three independent experiments; one-way analysis of variance followed by the least significant difference test). #P < 0.05, vs. control group; *P < 0.05, vs. OGD/R group (n = 3). SXT: Shuxuetong injection; OGD/R: oxygen-glucose deprivation/reperfusion; ICAM-1: intercellular adhesion molecule-1; VCAM-1: vascular cell adhesion molecule-1; TNF: tumor necrosis factor; IL: interleukin; iNOS: inducible nitric oxide synthase; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.