Table 1.
Genes | Primer/probe | Primer/probe sequences |
TNF-α | Forward primer | 5′-CGG GGT GAT CGG TCC CCA AAG-3′ |
Reverse primer | 5′-GGA GGG CGT TGG CGC GCT GG-3′ | |
IL-1β | Forward primer | 5′-GAC CTT CCA GGA TGA GGA CA-3′ |
Reverse primer | 5′-AGC TCA TAT GGG TCC GAC AG-3′ | |
IL-6 | Forward primer | 5′-CAG AGA TAC AAA GAA ATG ATG G-3′ |
Reverse primer | 5′-ACT CCA GAA GAC CAG AGG AAA-3′ | |
iNOS | Forward primer | 5′-CAC CTT GGA GTT CAC CCA GT-3′ |
Reverse primer | 5′-ACC ACT CGT ACT TGG GAT GC-3′ | |
GAPDH | Forward primer | 5′-CTT CAC CAC CAT GGA GAA GGC-3′ |
Reverse primer | 5′-GGC ATG GAC TGT GGT CAT GAG-3′ |
GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; TNF: tumor necrosis factor; IL: interleukin; iNOS: inducible nitric oxide synthase.