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. 2019 May;14(5):783–793. doi: 10.4103/1673-5374.249226

Table 1.

Primers used for real-time reverse transcription polymerase chain reaction

Genes Primer/probe Primer/probe sequences
TNF-α Forward primer 5′-CGG GGT GAT CGG TCC CCA AAG-3′
Reverse primer 5′-GGA GGG CGT TGG CGC GCT GG-3′
IL-1β Forward primer 5′-GAC CTT CCA GGA TGA GGA CA-3′
Reverse primer 5′-AGC TCA TAT GGG TCC GAC AG-3′
IL-6 Forward primer 5′-CAG AGA TAC AAA GAA ATG ATG G-3′
Reverse primer 5′-ACT CCA GAA GAC CAG AGG AAA-3′
iNOS Forward primer 5′-CAC CTT GGA GTT CAC CCA GT-3′
Reverse primer 5′-ACC ACT CGT ACT TGG GAT GC-3′
GAPDH Forward primer 5′-CTT CAC CAC CAT GGA GAA GGC-3′
Reverse primer 5′-GGC ATG GAC TGT GGT CAT GAG-3′

GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; TNF: tumor necrosis factor; IL: interleukin; iNOS: inducible nitric oxide synthase.