Figure 1.

WIPF2 and Arp2/3 mediate internalization of conidia into 1HAEo- cells. (A,B) 1HAEo- cells were infected with GFP-expressing A. fumigatus conidia for 15 min (A) or 1 h (B). After infection, samples were immunolabeled using anti-WIPF2 antibody and DAPI and imaged using super-resolution confocal microscopy. (C) 1HAEo-cells were transfected with siRNA targeting no known gene in the genome (NC-1) or WIPF2. The nystatin protection assay was used to assess the extent of internalization. P < 0.05 (Student's T-test). (D) 1HAEo-cells were treated with CK-666, an Arp2/3 inhibitor or DMSO vehicle before infection by GFP-expressing A. fumigatus conidia. Extracellular conidia were labeled by immunolabeling without permeabilization followed by manual counting. The proportion of observed conidia that were internalized is shown. P < 0.05 (Student's T-test).