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Characterization of soluble wt α-syn and α-synCC. (A) Far-UV CD spectra of wt α-syn (dashed line) and its double cysteine mutant (full line). Soluble proteins at 15 µM were measured in buffer A (black), B (blue) and C (green). (B) Bis-ANS fluorescence spectra of 10 µM bis-ANS bound to 15 µM soluble wt α-syn and α-synCC in buffer A, B and C.
