Fig. 6.

Characterization of wt α-syn and α-synCC aggregates formed after 5 day-incubation in buffer A, B and C. (A) Static light scattering of 10 µM wt α-syn (dashed line) and α-synCC aggregates (full line) in different buffers: A (black), B (blue) and C (green). (B) Fluorescence emission spectra of Th-T upon incubation with 10 µM wt α-syn and α-synCC fibrils formed in different buffers. (C) CR normalized absorbance spectra in the presence of 10 µM α-syn aggregates. Free CR absorbance spectrum is represented in grey. (D) TEM micrographs of negatively stained aggregates formed by wt α-syn (upper panel) and α-synCC (lower panel) in the three different conditions. Scale bars represent 200 nm. Images were taken at a 10,000× magnification. (E) PK degradation patterns of soluble wt α-syn and α-synCC (panels without frames), together with the digestion pattern of the aggregated forms (framed panels), monitored over time on Coomassie-stained SDS-PAGE. The time of digestion (in min) is indicated on the top of each panel. (F) The secondary structure of wt α-syn and α-synCC aggregates formed after 5 days. ATR-FTIR absorbance spectra in the amide I region was acquired and the fitted individual bands after Gaussian deconvolution are indicated.