Fig. 8.

wt α-syn aggregation in the presence of α-synCC. (A) Th-T time course of fibrillization of wt α-syn in buffer B, in the absence (black line) and in the presence of increasing concentrations of soluble α-synCC (lines coloured in a gradient of blue). (B) The first 800 min of the reaction showing the aggregation lag phase. (C) Bar diagram representation of the lag time of the aggregation kinetics in the presence of increasing α-synCC concentration. Error bars indicate ± SE (n = 3). (D) TEM micrographs of aggregates generated at the final point of the monitored aggregation kinetics. Scale bars correspond to 200 nm. Images were taken at a 10,000x magnification.