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. 2019 Feb 5;22:101135. doi: 10.1016/j.redox.2019.101135

Fig. 10.

Fig. 10

Evaluation of wt α-syn and α-synCC oligomers conformation and toxicity. (A) Cell viability of SH-SY5Y neuroblastoma cells after 72 h-incubation in the presence of different concentrations of soluble (S) and oligomeric (O) forms of wt α-syn and α-synCC. Error bars indicate ± SE (n = 3). (B) Electron micrographs of oligomeric assemblies of wt α-syn (dashed line) and α-synCC (full line) generated after 21 h of incubation in 20 mM HEPES, pH 7.4. The scale bars correspond to 200 nm. Images were taken at a 20,000x magnification. (C) Static light scattering of 10 µM wt α-syn (dashed line) and α-synCC (full line) oligomeric assemblies. (D) Bis-ANS fluorescence spectra of 10 µM bis-ANS bound to 15 µM wt α-syn and α-synCC oligomers in 20 mM HEPES, pH 7.4. (E) Secondary structure of wt α-syn (left panel) and α-synCC oligomers (right panel). ATR-FTIR absorbance spectra in the amide I region was acquired and the fitted individual bands after Gaussian deconvolution are indicated.