KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Cas9 (IF staining) | Active Motif | Cat#61577 (7A9-3A3) |
| Mouse monoclonal anti-Cas9 (ChIP) | Active Motif | Cat#61757 (8C1-F10) |
| Chicken polyclonal anti-GFP | Aves Labs | Cat#GFP-1020 |
| Chicken polyclonal anti-MAP2 | Encor Biotech | Cat# CPCA-MAP2 |
| Rabbit polyclonal anti-FMRP (WB, ICC, IHC) | Cell Signaling | Cat#4317 |
| Mouse monoclonal anti-FMRP (ICC, IHC) | Biolegend | Cat#834701 |
| Mouse monoclonal anti-FMR1PolyG | EMD Millipore | Cat#MABN784 |
| Goat polyclonal anti-mCherry | SICGEN | Cat#AB0040-200 |
| Mouse monoclonal anti-RNA Polymerase II | Abcam | Cat#ab817 |
| Rabbit polyclonal anti-H3K27Ac | Abcam | Cat#ab4729 |
| Rabbit polyclonal anti-H3K9me3 | Abcam | Cat#ab8898 |
| Mouse monoclonal anti-H3K27me3 | Abcam | Cat#ab6002 |
| Rabbit polyclonal anti-H3K4me3 | Millipore | Cat#07-473 |
| Chemicals | ||
| Doxycycline hyclate | Sigma Aldrich | Cat#D9891-100G |
| Critical Commercial Assays | ||
| EpiTect Bisulfite Kit | Qiagen | Cat#59104 |
| EpiNext™ High-Sensitivity Bisulfite-Seq kit | EPIGENTEK | Cat#P-1056 |
| EpiNext™ NGS Barcode Set-12 | EPIGENTEK | Cat#P-1060 |
| DNeasy Blood & Tissue Kit | Qiagen | Cat#69504 |
| Zymoclean Gel DNA Recovery Kit | Zymo Research | Cat#D4002 |
| DNA Clean & Concentrator-5 | Zymo Research | Cat#D4013 |
| X-tremeGENE™ 9 DNA Transfection Reagent | Sigma Aldrich | Cat#6365809001 |
| Direct-zol RNA Miniprep | Zymo Research | Cat#R2050 |
| SuperScript III First-Strand Synthesis SuperMix | Life Technologies | Cat#18080400 |
| Fast SYBR Green Master Mix | Life Technologies | Cat#4385618 |
| Deposited Data | ||
| Raw data files for sequencing | NCBI Gene Expression Omnibus | Database: GSE102655, GSE102684, GSE108171, GSE108498, GSE108577 |
| Experimental Models: Organisms/Strains | ||
| Mouse: NOD-scid IL2Rgnull, | Jackson Laboratories | Stock No: 005557 |
| Experimental Models: Cell Lines | ||
| FX52 iPSCs | (Urbach et al., 2010) | N/A |
| FXS iPSC (135.3) | (Brick et al., 2014) | N/A |
| FXS iPSC (FXS_SW) | (Xie et al., 2016) | N/A |
| Recombinant DNA | ||
| Fuw-dCas9-Tet1-P2A-BFP | This paper | N/A |
| pgRNA | This paper | N/A |
| Fuw-AcrIIA4-P2A-GFP | This paper | N/A |
| See “Plasmid design and construction” in METHOD DETAILS section | ||
| Sequence-Based Reagents | ||
| See Table S4, S5, S6 for primer sequences | This paper | N/A |
| Software and Algorithms | ||
| MACS (ChIP-seq algorithms) | Wu et al., 2014b | http://liulab.dfci.harvard.edu/MACS/ |
| ImageJ (Fiji) | NIH | http://imagej.net/Fiji |