. 2019 Feb 13;10:54. doi: 10.1186/s13287-019-1159-3

Table 1.

Primer information in the qRT-PCR assay

Target gene	Primer sequence (5′-3′)		Annealing temperature (°C)	Product size (bp)
ACTB	Sense	AGAGCTACGAGCTGCCTGAC	60	270
ACTB	Antisense	AGCACTGTGTTGGCGTACAG	60	270
SFRP2	Sense	ACGGCATCGAATACCAGAACA	58	176
SFRP2	Antisense	CTCGTCTAGGTCATCGAGGCA	58	176
NETO2	Sense	AGATGGGCCATTTGGTTTCTC	56	139
NETO2	Antisense	TGCTCGAAATCCCAGTCCTTC	56	139
SULF2	Sense	CACTGGCAAGTACGTCCACAA	60	111
SULF2	Antisense	CTATTGAGGTACACGGCAAAGG	60	111
COL8A1	Sense	GGGAGTGCTGCTTACCATTTC	56	75
COL8A1	Antisense	AGCGGCTTGATCCCATAGTAG	56	75
NRK	Sense	CATTGGCCTTGGTACTTATGGC	59	93
NRK	Antisense	GTCTTACGAGCGTTCATCACTT	59	93
SYTL2	Sense	GCCCAGTGTAAGGACTTAGCA	60	87
SYTL2	Antisense	GCCTTTGTCTGGTAGCAAATAGG	60	87
CMKLR1	Sense	CAGTTACGGTGATGAATACCCTG	60	115
CMKLR1	Antisense	GACGATGCTGTAGACCACCAC	60	115

bp base pair