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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: J Leukoc Biol. 2018 Feb 13;103(5):919–932. doi: 10.1002/JLB.3A0617-252RR

FIGURE 3. PLD deficiency impacts alveolar barrier disruption.

FIGURE 3

(A) Total lung PLD activity in wild-type (WT) mice, and mice deficient in Pld1 and Pld2 gene, at t = 0 and t = 24 h after intrabronchial HCl. Results are expressed as mean ± SEM, N ≥ 10, *P < 0.05. (B) Albumin levels in BAL fluid from WT, Pld1−/−, and Pld2−/− animals 24 h after intrabronchial HCl. (C) Evans blue dye levels in left lung homogenate 24 h after intrabronchial HCl and treatment with a selective inhibitor of PLD1 (PLD1in, black) or PLD2 (PLD2in, gray), or vehicle (Veh, hatched). Results are expressed as mean ± SEM, N ≥ 10, *P < 0.05. (D) Representative lung sections from WT (injured left lung and uninjured right lung from same animal), Pld1−/−, and Pld2−/− animals