Table 5.

Estimated differences in cognitive test scores at age7–11 y in association with a 10% increase in methylation of LAMB2 CpG sites in cord blood.

CpGd	Model 1a			Model 2b,c
	N	Estimate (95% CI)	P-value	N	Estimate (95% CI)	P-value
KBIT-2 verbal test
cg05654765	415	0.70 ($\mathrm{-1}\mathrm{.39}$, 2.78)	0.51	382	0.49 ($\mathrm{-1}\mathrm{.68}$, 2.67)	0.66
cg14099457	415	0.70 ($\mathrm{-1}\mathrm{.17}$, 2.58)	0.46	382	0.67 ($\mathrm{-1}\mathrm{.29}$, 2.62)	0.50
cg03732535	415	0.88 ($\mathrm{-4}\mathrm{.86}$, 6.62)	0.76	382	1.04 ($\mathrm{-5}\mathrm{.02}$, 7.10)	0.74
cg02954987	415	0.54 ($\mathrm{-0}\mathrm{.74}$, 1.81)	0.41	382	0.41 ($\mathrm{-0}\mathrm{.90}$, 1.71)	0.54
KBIT-2 nonverbal test
cg05654765	418	$\mathrm{-2}\mathrm{.74}$ ($\mathrm{-5}\mathrm{.29}$, $\mathrm{-0}\mathrm{.20}$)	0.03	385	$\mathrm{-2}\mathrm{.78}$ ($\mathrm{-5}\mathrm{.47}$, $\mathrm{-0}\mathrm{.10}$)	0.04
cg14099457	418	$\mathrm{-2}\mathrm{.28}$ ($\mathrm{-4}\mathrm{.57}$, 0.00)	0.05	385	$\mathrm{-2}\mathrm{.43}$ ($\mathrm{-4}\mathrm{.84}$,$\mathrm{-0}\mathrm{.02}$)	0.05
cg03732535	418	$\mathrm{-10}\mathrm{.89}$ ($\mathrm{-17}\mathrm{.85}$,$\mathrm{-3}\mathrm{.94}$)	0.002	385	$\mathrm{-10}\mathrm{.32}$ ($\mathrm{-17}\mathrm{.75}$, $\mathrm{-2}\mathrm{.88}$)	0.007
cg02954987	418	$\mathrm{-1}\mathrm{.45}$ ($\mathrm{-3}\mathrm{.00}$, 0.11)	0.07	385	$\mathrm{-1}\mathrm{.55}$ ($\mathrm{-3}\mathrm{.16}$, 0.07)	0.06
WRAML2 picture memory standard score
cg05654765	417	0.02 ($\mathrm{-0}\mathrm{.46}$, 0.50)	0.93	384	$\mathrm{-0}\mathrm{.07}$ ($\mathrm{-0}\mathrm{.58}$, 0.44)	0.79
cg14099457	417	0.06 ($\mathrm{-0}\mathrm{.38}$, 0.49)	0.80	384	$\mathrm{-0}\mathrm{.005}$ ($\mathrm{-0}\mathrm{.46}$, 0.45)	0.98
cg03732535	417	$\mathrm{-0}\mathrm{.08}$ ($\mathrm{-1}\mathrm{.41}$, 1.25)	0.90	384	$\mathrm{-0}\mathrm{.29}$ ($\mathrm{-1}\mathrm{.70}$, 1.13)	0.69
cg02954987	417	$\mathrm{-0}\mathrm{.04}$ ($\mathrm{-0}\mathrm{.33}$, 0.26)	0.80	384	$\mathrm{-0}\mathrm{.04}$ ($\mathrm{-0}\mathrm{.35}$, 0.26)	0.79
WRAML2 design memory standard score
cg05654765	416	0.13 ($\mathrm{-0}\mathrm{.31}$, 0.56)	0.57	383	0.06 ($\mathrm{-0}\mathrm{.40}$, 0.51)	0.80
cg14099457	416	0.16 ($\mathrm{-0}\mathrm{.23}$, 0.55)	0.42	383	0.13 ($\mathrm{-0}\mathrm{.28}$, 0.54)	0.54
cg03732535	416	0.95 ($\mathrm{-0}\mathrm{.25}$, 2.14)	0.12	383	0.76 ($\mathrm{-0}\mathrm{.51}$, 2.02)	0.24
cg02954987	416	0.20 ($\mathrm{-0}\mathrm{.07}$, 0.46)	0.14	383	0.15 ($\mathrm{-0}\mathrm{.12}$, 0.43)	0.27
WRAML2 visual memory standard score
cg05654765	415	0.15 ($\mathrm{-0}\mathrm{.52}$, 0.83)	0.65	382	0.00 ($\mathrm{-0}\mathrm{.72}$, 0.71)	0.99
cg14099457	415	0.22 ($\mathrm{-0}\mathrm{.39}$, 0.83)	0.48	382	0.13 ($\mathrm{-0}\mathrm{.51}$, 0.76)	0.70
cg03732535	415	0.88 ($\mathrm{-0}\mathrm{.98}$, 2.74)	0.35	382	0.48 ($\mathrm{-1}\mathrm{.51}$, 2.47)	0.63
cg02954987	415	0.16 ($\mathrm{-0}\mathrm{.25}$, 0.57)	0.44	382	0.11 ($\mathrm{-0}\mathrm{.32}$, 0.54)	0.61
WRAVMA standard score
cg05654765	414	0.90 ($\mathrm{-1}\mathrm{.72}$, 3.52)	0.50	381	0.94 ($\mathrm{-1}\mathrm{.81}$, 3.69)	0.50
cg14099457	414	0.29 ($\mathrm{-2}\mathrm{.06}$, 2.65)	0.81	381	0.18 ($\mathrm{-2}\mathrm{.29}$, 2.65)	0.89
cg03732535	414	3.33 ($\mathrm{-3}\mathrm{.89}$, 10.55)	0.37	381	3.86 ($\mathrm{-3}\mathrm{.81}$, 11.54)	0.32
cg02954987	414	0.61 ($\mathrm{-0}\mathrm{.98}$, 2.21)	0.45	381	0.43 ($\mathrm{-1}\mathrm{.21}$, 2.08)	0.60

^aModel 1: Adjusted for maternal age at enrollment, race/ethnicity (white, black, other), smoking during pregnancy (any, former, never), education (any college, no college), child’s age at testing (continuous, in years), and child’s sex.

^bModel 2: Additionally adjusted for maternal alcohol consumption during the first trimester of pregnancy (servings per day, continuous) and median neighborhood household income.

^cModel 2 had 33 more missing values than model 1 due to the additional adjustment of alcohol consumption and median household income.

^dThe CpG sites evaluated had significantly higher methylation in cord blood ($P_{BH}<0.05$) in association with a 50% reduction in residential proximity to major roadways at birth.