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| Factors | Symbols | Coded levels | ||
|---|---|---|---|---|
| -1 | 0 | +1 | ||
| Temperature (oC) | X1 | 25 | 30 | 35 |
| Nitrogen sources (%)* | X2 | 6 | 15 | 24 |
| Glucose (%) | X3 | 10 | 20 | 30 |
* The nitrogen source was composed by peptone and yeast extract. (Peptone: Yeast extract = 2:1)
