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. 2019 Feb 14;14(2):e0211916. doi: 10.1371/journal.pone.0211916

Fig 5. Treatment with salinomycin results in accumulation of dysfunctional mitochondria and increased generation of ROS.

Fig 5

(A) Analysis of total mitochondrial mass in HT29, SW480, and HCT116 cells after exposure to increasing concentrations of salinomycin (0.1, 0.5, 2, 5, and 10 μM) was assessed using MTR green. After 24 hours, accumulation of mitochondrial mass was observed in all three cell lines. (B) Accumulation of dysfunctional mitochondria was further visualized applying MRT green immunostaining after 24 hours of treatment. (C) Increased generation of ROS in HT29, SW480, and HCT116 cells after treatment with salinomycin using CM-H2DCFDA staining and analyzed by flow cytometry. (D) Immunostaining of HT29, SW480, and HCT116 cells after exposure of salinomycin confirmed increased ROS generation. Results are displayed as a summary of at least three independent experiments as mean ± SD or as representative image capture by fluorescence microscopy; * p < 0.05 compared with control. N = 3 individual experiments. Scale bars = 50 μM.