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. 2019 Feb 14;14(2):e0212202. doi: 10.1371/journal.pone.0212202

Fig 6. Overexpressed Rap2b increases the recruitment of LC3 to the C. burnetii vacuole.

Fig 6

A. CHO cells were co-transfected with RFP-LC3 and pEGFP (control), wt pEGFP-Rap2b or pEGFP-Rap2b ΔAAX and subsequently infected with C. burnetii for 48 hours (MOI = 10). Cells were fixed and processed for fluorescence microscopy. B. Fluorescence intensity along the yellow line depicted in the insets of panel A. C. Quantification of the number of LC3 vacuoles in cells transfected with the indicated constructs in A. D. Quantification of the vacuole number in cells transfected with the indicated constructs. E. Quantification of the vacuole diameter in cells transfected with the indicated constructs. The data represent the mean ± SEM of at least 2 independent experiments. Tukey test * p ≤ 0.05. Bars = 10 μm.