Table 1. Bacterial strains, and plasmids used in this study.
| Strains | Characteristic(s) | Source or reference |
|---|---|---|
| E. coli DH5α | F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ | Thermo Fisher Scientific, USA |
| E. coli S17.1 | recA thi pro hdsR4 (rK- mK+) (RP4-2T::M-Km::Tn7) Tpr Smr λpir | [17] |
| A. brasilense Sp7 | Wild-type strain | [18] |
| A. brasilense 2112 | A. brasilense ipdC::Sp-lacZ- KmR isogenic of Sp7 strain | [8] |
| A. brasilense 2116 | A. brasilense tyrR::GmR isogenic of Sp7 strain | This study |
| A. brasilense 2117 | A. brasilense tyrR::GmR (pJBtyrR) | This study |
| A. brasilense 2118 | A. brasilense 2116 (pJB3Tc20) | This study |
| A. brasilense 2119 | A. brasilense tyrR::GmR ipdC::Sp-lacZ- KmR isogenic of Sp7 strain | This study |
| Plasmids | ||
| pCR2.1-TOPO | Cloning vector, ApR, KmR. | Invitrogen Thermo Fisher MX |
| pBlueScript + (pBSK+) | Cloning vector | Stratagene, La Jolla, CA, USA |
| pBSL142 | Plasmid vector GmR | [19] |
| pJB3Tc20 | Plasmid derived from RK2 oriT, TcR, ApR | [20] |
| pSUP202 | Suicide plasmid TcR, ApR, CmR | [17] |
| pCR2.1-tyrR | Plasmid derived from pCR2.1-TOPO carrying a fragment of 2886 bp with the tyrR gene, ApR, KmR. | This study |
| pBSK-tyrR-Sp7 | Plasmid derived from pBSK+ carrying the tyrR gene in a fragment of 1964 bp | This study |
| pCR2.1-tyrR::GmR | Plasmid derived from pCR2.1-tyrR, with tyrR::GmR | This study |
| pAB2116 | Plasmid derived from pSUP202 carrying tyrR::GmR in a fragment of 3.5 kb | This study |
| pJB3-tyrR-Sp7 | Plasmid derived from pJB3Tc20 carrying the tyrR gene in a fragment of 1542 bp | This study |
Gm = Gentamycin, Ap = Ampicillin, Tc = Tetracycline, Cm = Chloramphenicol, Km = Kanamycin.