Fig 5. Static deactivation of pol V Mut wt at 37°C and 30°C.

Static deactivation of pol V Mut wt was measured as shown in Fig 4A. Pol V Mut wt deactivates statically in the absence of DNA synthesis at 37°C (A-B) and 30°C (C-D). Pol V Mut wt was incubated either alone (I. and black circles in the graphs) or with ATPγS (II. and white circles in the graphs), or with ATPγS and 12nt oh HP DNA (III. and black triangles in the graphs). The static deactivation conditions and analysis are described in the legend for Fig 4. Pol V Mut wt deactivates more slowly at 30°C (C-D) compared to 37°C (A-B). However, in contrast to pol V Mut E38K/ΔC17 (Fig 4), the degree of stabilization with ATPγS is much less for pol V Mut wt. Deactivated pol V Mut wt is reactivated by incubation with RecA* (+RecA*).