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. 2019 Feb 4;15(2):e1007591. doi: 10.1371/journal.ppat.1007591

Fig 4. Rv0500A binds directly to the rv2390c promoter.

Fig 4

(A and B) Rv0500A binds directly to the rv2390c promoter but not the kdpF promoter. Electrophoretic mobility shift assays (EMSAs) were performed using purified recombinant N-terminally 6x-His-tagged Rv0500A and DNA probes for the rv2390c promoter (A) or the kdpF promoter (B). The highest concentration binding reaction contained 0.1 μM His-Rv0500A followed by two-fold serial dilutions. A control with no His-Rv0500A protein added is shown in the first lane. All DNA probes were labeled with IRDye 700. Data are representative of 2–3 independent experiments. (C) Rv0500A binds directly to a 3’ section of the rv2390c promoter. The schematic at the top shows the “split” promoter regions. EMSAs were performed as above with the 5’ (left panel) or 3’ (right panel) sections of the rv2390c promoter. Data are representative of 2 independent experiments.