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. 2019 Feb 4;15(2):e1007591. doi: 10.1371/journal.ppat.1007591

Fig 6. Intraphagosomal K+ concentration increases during phagosomal maturation.

Fig 6

(A and B) APG4/AF594 beads respond specifically to [K+]. APG4/AF594 beads were placed in buffer containing specific [K+] or [Na+] (A), or in buffer with 100 mM Bis-Tris adjusted to the indicated pH with HCl (B). APG4 and AF594 fluorescence were measured on a microplate reader, and data are shown as means ± SD from 4 wells, representative of 2–3 independent experiments. (C and D) Intraphagosomal [K+] increases during phagosomal maturation independent of an oxidative burst. APG4/AF594 beads were added to resting or activated murine bone marrow-derived macrophages (MØ) from WT (C and D) or gp91phox-/- mice (D), and fluorescence tracked with a microplate reader over time. Sensor beads were also added to wells containing only media, with no macrophages (“beads only, no MØs”). Data are shown as means ± SD from 4 wells, representative of 3–5 independent experiments.