Table 1. Mutant operator sequences.
The listed operator sequences were used to evaluate energy matrix predictions. They are mutated relative to the O1 lac operator. The predicted binding energy was generated using the matrix with an O1 reference sequence with R = 130 LacI tetramers in the background strain.
| Sequence | Predicted ΔεR (kBT) | Measured ΔεR (kBT) |
|---|---|---|
| 1 bp mutants: | ||
| AATTGTGAGCGGAGAACAATT | -12.63 | -12.24 |
| AATTGTGAGCGCATAACAATT | -15.71 | -15.30 |
| AATTGTGAGCGGATCACAATT | -15.22 | -14.99 |
| AATTGTGAGCGGAAAACAATT | -12.91 | -12.50 |
| AATTGCGAGCGGATAACAATT | -12.14 | -11.30 |
| AATTGTGAGGGGATAACAATT | -13.16 | -12.35 |
| AATTGTGAGCGGATATCAATT | -13.66 | -13.29 |
| AATTGTGAGCAGATAACAATT | -11.11 | -10.25 |
| AATTGTGAGAGGATAACAATT | -8.89 | -10.00 |
| 2 bp mutants: | ||
| AATTGTGAGCGGGTAACAACT | -13.82 | -14.79 |
| AAATGTGAGCGGATAACAACT | -13.61 | -14.40 |
| AATTGTGAGCGAGTAACAATT | -14.36 | -15.12 |
| ATTTGTGAGCGGAGAACAATT | -12.55 | -11.52 |
| CATTGTGAGCGCATAACAATT | -15.34 | -14.80 |
| AATTGTGAGCGGAACACAATT | -12.83 | -13.31 |
| AATTGTGAGCGGAATACAATT | -11.70 | -12.03 |
| AATTGCGAGCGGATAACAAAT | -12.06 | -10.78 |
| AATTGTGAGGGGATAACAATC | -14.13 | -12.15 |
| 3 bp mutants: | ||
| AAATGTGAGCGAGTAACAATT | -13.84 | -14.57 |
| AATTGTGAGCGAGTAACAACT | -13.19 | -14.67 |
| ATTTGTGAGCGAAGAACAATT | -11.92 | -10.83 |
| CATTGTGAGCGCATAACATTT | -15.39 | -14.18 |
| AATTGTGAGCGGAACACAATG | -13.72 | -12.17 |
| AATTGTGAGCGGGATACAATT | -11.39 | -11.86 |
| AATTGCGAGCGGATAACAAAG | -12.96 | -10.62 |
| AATTGTGAGGGTATAACAATC | -14.10 | -11.79 |