Table 2.

Mutations in the cII Gene of Livers from the Riddelliine-Treated and Control Big Blue Rats

positiona	mutation b	amino acid change	sequence context 5′ → 3′c	no. of mutations (independent)		position a	mutation b	amino acid change	sequence context 5′ → 3′c	no. of mutations (independent)
				control	treated					control	treated
(−14)-(−13)	GG → TT	N/A	ctaAGGaaa		1	134 −135	GG → TT	Arg → Ile	aagAGGgac		1
–13	G → T	N/A	ctaAGGaaa		3 (3)	135	G → A	Arg → Arg	aagAGGgac		1
19	C → G	Arg → Gly	aaaCGCaac		1	135	G → T	Arg → Ser	aagAGGgac		1
19	C → T	Arg → Cys	aaaCGCaac	1		136	G → T	Asp → Tyr	aggGACtgg		1
24	C → G	Asn → Lys	cgcAACgag	1		139	T → G	Trp → Gly	gacTGGatt		1
27	G → T	Glu → Asp	aacGAGgct		1	145	C → A	Pro → Thr	attCCAaag		1
28	G → A	Ala → Thr	gagGCTcta	1		145	C → T	Pro → Ser	attCCAaag		1
29	C → T	Ala → Val	gagGCTcta		3 (2)	152	T → G	Phe → Cys	aagTTCtca		2 (2)
30	T → A	Ala → Ala	gagGCTcta		1	154	T → C	Ser → Pro	ttcTCAatg	1
34	C → T	Arg → stop	ctaCGAatc	4 (3)	1	163	C → T	Leu → Phe	ctgCTTgct		2 (1)
35	G → A	Arg → Gln	ctaCGAatc	1		164	T → C	Leu → Pro	ctgCTTgct		1
40	G → A	Glu → Lys	atcGAGagt	1		166	G → A	Ala → Thr	cttGCTgtt	1
46	G → T	Ala → Ser	agtGCGttc		1	172	C → A	Leu → Ile	gttCTTgaa		1
57/62	+ A	frameshift	aacAAAatc	1		175	G → C	Glu → Gln	cttGAAtgg		1
60	A → T	Lys → Asn	aacAAAatc		1	178/185	+ G	frameshift	gaaTGGGGGGTCgtt	6 (4)	7 (5)
61	A → C	Ile → Leu	aaaATCgca		1	178	T → C	Trp → Arg	gaaTGGggg	1
64	G → C	Ala → Pro	atcGCAatg		1	179	G → T	Trp → Leu	gaaTGGggg		1
65	C → T	Ala → Val	atcGCAatg	1		179/184	− G	frameshift	gaaTGGGGGGTCgtt	2 (2)	1
65/68	+ A	frameshift	gcaATGctt		1	180	G → A	Trp → stop	gaaTGGggg	1
73–74	GG → AT	Gly → Ile	cttGGAact		2 (2)	180 −181	GG → AT	Trp-Gly → stop-Trp	gaaTGGGGGgtc		1
73–74	GG → TT	Gly → Leu	cttGGAact		1	182	G → T	Gly → Val	tggGGGgtc		2 (2)
74	G → T	Gly → Val	cttGGAact		4 (4)	184	G → T	Val → Phe	gggGTCgtt		1
76	A → G	Thr → Ala	ggaACTgag		1	185	T → G	Val → Gly	gggGTCgtt	1
81	G → A	Glu → Glu	actGAGaag	1		185, 178/186	T → G;+G	complex	gggGTCgtt	1
86	C → A	Thr → Lys	aagACAgcg		1	187	G → T	Val → Phe	gtcGTTgac		1
86	C → G	Thr → Arg	aagACAgcg	1		191	A → G	Asp → Gly	gttGACgac		1
89	C → A	Ala → Glu	acaGCGgaa	1		192	C → G	Asp → Glu	gttGACgac		1
89	C → T	Ala → Val	acaGCGgaa	3 (3)	4 (2)	196	G → A	Asp → Asn	gacGACatg	2 (2)	1
90–91	GG → TT	Ala-Glu → Ala-stop	acaGCGGAAgct		1	203	C → A	Ala → Asp	atgGCTcga		1
91	G → T	Glu → stop	gcgGAAgct		1	206	G → A	Arg → Gln	gctCGAttg	1	3 (2)
94	G → A	Ala → Thr	gaaGCTgtg		1	208	T → A	Leu → Met	cgaTTGgcg	1
95	C → T	Ala → Val	gaaGCTgtg		1	209	T → G	Leu → Trp	cgaTTGgcg	1
98	T → C	Val → Ala	gctGTGggc	1		211	G → A	Ala → Thr	ttgGCGcga	1
99/101	− G	frameshift	gctGTGGGCgtt	1		212	C → A	Ala → Glu	ttgGCGcga		1
101	G → A	Gly → Asp	gtgGGCgtt	1		212	C → T	Ala → Val	ttgGCGcga	2 (2)	2 (2)
101	G → T	Gly → Val	gtgGGCgtt		1	212/214	+ G	frameshift	ttgGCGCGAcaa		1
103	G → A	Val → Ile	ggcGTTgat	4 (3)	3 (2)	213	G → T	Ala → Ala	ttgGCGcga	1
103	G → T	Val → Phe	ggcGTTgat		1	214	C → T	Arg → stop	gcgCGAcaa	9 (5)	3 (2)
113	C → A	Ser → stop	aagTCGcag		1	217	C → T	Gln → stop	cgaCAAgtt		1
118	A → T	Ile → Phe	cagATCagc		1	220	G → T	Val → Phe	caaGTTgct	1
122	G → A	Ser → Asn	atcAGCagg	1		222	T → G	Val → Val	caaGTTgct		1
125	G → T	Arg → Met	agcAGGtgg		1	224	C → A	Ala → Asp	gttGCTgcg	1
126	G → T	Arg → Ser	agcAGGtgg	1		230	T → G	Ile → Ser	gcgATTctc	1
127	T → A	Trp → Arg	aggTGGaag	1		232	C → A	Leu → Ile	attCTCacc		2 (2)
128	G → A	Trp → stop	aggTGGaag		1	233	T → C	Leu → Pro	attCTCacc		1
129	G → T	Trp → Cys	aggTGGaag		1	266	G → A	Arg → His	gagCGTtct		1
131	A → T	Lys → Met	tggAAGagg	1		total				63 (55)	92 (83)
133	A → T	Arg → Trp	aagAGGgac		1

^aPosition 1 is the first base of the start codon in the cII coding sequence.

^bPresented in terms of sequence change on a nontranscribed DNA strand.

^cUppercase indicates the target codon, and target bases are underlined. Abbreviations: −, deletion; +, insertion.