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. Author manuscript; available in PMC: 2019 Feb 14.
Published in final edited form as: Methods Mol Biol. 2019;1911:395–419. doi: 10.1007/978-1-4939-8976-8_27
Reagent 1 Rxn (μL)
5x Phusion buffer 10
10 mM dNTP mix 1
Phusion polymerase 1
HuJH-F 2
Gap3
Plasmidsa (350 ng) 2
Molecule grade water 34
Final vol 50
a

Plasmids pYD2.A2H.Vκ and pYD2.A2H.Vλ from steps 1 and 2