Table 2.
MHC Class-II-Bound Salmonella Peptides Identified Using Immunoproteomics
| protein | name | peptide | start residue | end residue |
|---|---|---|---|---|
| Peptide Score >40 | ||||
| E1WFF7 | glyceraldehyde-3-phosphate dehydrogenase | TGMAFRVPTPNVSVVDLT (31 peptides) | 227 | 244 |
| E1WCL8 | 3-oxoacyl-[acyl-carrier-protein] synthase I | LEHGFIAPSINIEELD | 350 | 365 |
| E1WA22 | flagellin | IDGKTYNASKAAGHDF | 387 | 402 |
| AVQNRFNSAITNLGNTVN | 439 | 456 | ||
| Peptide Score 30–40 | ||||
| E1W826 | dihydrolipoyl dehydrogenase | AGKKHYFDPKVIPSIAY (2 peptides) | 336 | 352 |
| E1WHK9 | phosphoglycerate kinase | LEFVEGKVLPAVA | 367 | 379 |
| E1WCC4 | glycerol-3-phosphate dehydrogenase | DPQQARIIEPSVNPALIG | 122 | 139 |
| E1WD28 | L-asparginase | GELGVQTLINAVPE | 52 | 65 |
| E1W8P7 | pyrroline-5-carboxylate reductase | GGAEVIAEPMIHPVVG | 155 | 170 |
| E1WF20 | 60 kDa chaperonin (GroEL protein) | GPKGRNVVLDKSFGAPTITKDGV (2 peptides) | 32 | 55 |
| Peptide Score 20–30 | ||||
| E1WFF1 | uncharacterized protein | KEPENSSIYSKMRVYDG | 324 | 340 |
| E1WE27 | 5-methyltetrahydro-pteroyltriglutamate--homocysteine methyltransferase | LLEEVDEALALG | 143 | 154 |
| E1W970 | hypothetical membrane protein | FKDPILGLVAGIQLSANDML | 144 | 163 |
| E1WH74 | fructose-bisphosphate aldolase class I | DLGDAVRTAVINKRAGGMGL | 298 | 317 |
| E1WFF7 | glyceraldehyde-3-phosphate dehydrogenase | KLTGMAFRVPTPNVSVVD | 225 | 242 |
| GMAFRVPTPNVS | 228 | 239 | ||
| E1WHM6 | biosynthetic arginine decarboxylase | ELMAVLAHAGMTRSVI | 133 | 148 |
| E1WC68 | TonB protein | PVVEPEPEPEPEPIPEPPK | 67 | 85 |
| E1W8I9 | hypothetical permease/MSF transporter | ALSMLIICMMVGAFLTLI | 302 | 319 |
| E1WIA3 | RNA polymerase sigma-54 factor | LDTADALEQKEMPEELPL | 67 | 84 |
| E1WCH9 | hypothetical membrane protein | AIAAWFMLLF | 398 | 407 |
| E1W9R3 | galactose-1-phosphate uridylyltransferase | VGYEMLAETQRDLTAEQAAERL | 314 | 335 |
| E1WGX3 | glycerol dehydratase large subunit | FIARYGINLNRAEEVMAM | 65 | 82 |
| E1W953 | outer membrane usher protein FimD | GTVIAGLPAGLTAYGGTQLA | 387 | 406 |
| E1WD27 | glutathione reductase | VPKKVMWHAAQIREAIH | 48 | 64 |
| E1W7R3 | Na(+)/H(+) antiporter NhaA | GVSLQGVTIDGLTSML | 273 | 288 |
| E1W9Y1 | molybdopterin biosynthesis MoeB protein | INPHITITPVNARLDDDAM (2 peptides) | 97 | 115 |
| E1WEE9 | transcriptional repressor | PTAVFCHSDVMAL | 246 | 258 |
| E1WBX7 | hypothetical exported protein | RMPQHDPALLRVQNISSSEL | 706 | 725 |
| E1WHT5 | hydrogenase-2 large subunit | DDVGPYEQSLVGTPIADPAK | 513 | 532 |
| E1WE91 | hypothetical 1 formate dehydrogenase-O, major subunit | NIRTMAMIQLLLGNMGMAGGGVNALR | 420 | 445 |
| E1WDV3 | aspartate--ammonia ligase | RGEMPQTIGGGIGQSRL | 284 | 300 |
| E1W898 | UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase) | CMIGGASVINGHMEI | 265 | 279 |
| E1WDR4 | chromosomal replication initiator protein DnaA | KADENDIRLPG | 308 | 318 |
| E1WBS8 | gamma-aminobutyraldehyde dehydrogenase | PALAAGNCVVIKPSEIT | 168 | 184 |
| E1WHM1 | hypothetical oxidoreductase | LTGVLMGANFSNHIVRM | 238 | 254 |
| E1W9N8 | TolQ protein | AFIALGAVKQATLQMVAPGIAEAL | 152 | 175 |
| E1W8V3 | cytochrome o ubiquinol oxidase subunit II | VAKAKQSPNTMNDMAAFEKVAMPSEYN | 235 | 261 |
| E1W9H9 | Pts system, N-acetylglucosamine-specific IIABC component | KAMVTINPEINMGVLAGIITG | 85 | 105 |
| E1WID9 | possible membrane transport protein | LMPLVFAAALGGNLSLIGA | 135 | 153 |
| E1WER9 | maltose transport inner membrane protein | LQLKETDALPGGERANLRII | 157 | 176 |
| E1WDG8 | DNA ligase B | KYPPVAQVAQVSAIQFSVG | 305 | 323 |
| E1W9T0 | uncharacterized protein | GSGLVAVP | 81 | 88 |
| E1W7W4 | 4-hydroxythreonine-4-phosphate dehydrogenase | LVVQLAQRAWPIELVVCADGA | 21 | 41 |
| E1W7F5 | sodium/proline symporter | KQYGWLDLYEIIPGFI | 445 | 460 |
| E1WEV1 | large repetitive protein | SAWTDGDYTLTVTVKDEAGN | 1988 | 2007 |
| E1W9E9 | leucine--tRNA ligase | YPSGRLHMGHVRNYT | 43 | 57 |
| H8WV08 | conjugal transfer protein | MLHDSINKLGEAIKKDEE | 121 | 138 |
| E1WJB0 | exodeoxyribonuclease VIII | LRPAMDFAKRIIAEDRED | 315 | 332 |
| E1WB21 | superfamily I DNA helicase | RKDLKTAETKIYTLHGV | 1003 | 1019 |
| E1WEI8 | pantothenate kinase | MHRLVKFVSDLKSGVP | 153 | 168 |
| E1WGH6 | tyrosine-specific transport protein | FSVTLGLLIGLWALMCYTA | 33 | 51 |
| E1WG19 | hypothetical exported protein | RKNASLFGNVLMGLGLVVMVVGVGYS | 3 | 28 |
| E1WHQ4 | hypothetical membrane protein | GINGAVAKIQQL | 78 | 89 |