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. Author manuscript; available in PMC: 2019 Feb 14.
Published in final edited form as: Cell Rep. 2018 Dec 11;25(11):3215–3228.e9. doi: 10.1016/j.celrep.2018.11.037

Figure 1. Generation of FOXF1+ Splanchnic Mesoderm from Human iPSCs.

Figure 1.

(A) Treatment of iPSCs in mesoderm induction medium (MIM) from day 1 to day 5 increases the expression of mesoderm-specific transcripts, as determined by qPCR. Means ± SDs shown.

(B) iPSCs differentiated in MIM show predominant expression of FOXF1 transcript (day 5, qPCR), indicative of splanchnic mesoderm formation Means ± SDs of three replicates shown.

(C) iPSCs differentiated in MIM induce expression of FOXF1 protein, as determined by western blot. β-Tubulin is the loading control.

(D) Treatment with MIM results in >95% FOXF1+ cells (red), as determined by immunofluorescence staining (n = 3, 20× images, representative image shown). Scale bar, 100 μm. Counterstaining with DAPI (blue).