Factor | stock concentration | working concentration | for 10 ml of medium |
---|---|---|---|
DMEM | 1X | 1X | 9 ml |
FBS | 10X | 1X | 1 ml |
Insulin | (10 μg/ml = 1.7 mM) | 170 nM | 1 μl |
T3 | 1 μM | 2 nM | 20 μl |
Rosiglitazone | 5 mM | 1 μM | 2 μl |
SB | 10 mM | 5 μM | 5 μl |
IBMX | 500 mM | 0.5 mM | 10 μl |
Dexamethasone | 5 mM | 5 μM | 10 μl |
Indomethacin | 125 mM | 125 μM | 10 μl |
AA2P | 50 mg/ml | 50 μg/ml | 10 μl |