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. Author manuscript; available in PMC: 2019 Feb 14.
Published in final edited form as: Cell Rep. 2018 Dec 11;25(11):3215–3228.e9. doi: 10.1016/j.celrep.2018.11.037
Factor stock concentration working concentration for 10 ml of medium
DMEM 1X 1X 9 ml
FBS 10X 1X 1 ml
Insulin (10 μg/ml = 1.7 mM) 170 nM 1 μl
T3 1 μM 2 nM 20 μl
Rosiglitazone 5 mM 1 μM 2 μl
SB 10 mM 5 μM 5 μl
IBMX 500 mM 0.5 mM 10 μl
Dexamethasone 5 mM 5 μM 10 μl
Indomethacin 125 mM 125 μM 10 μl
AA2P 50 mg/ml 50 μg/ml 10 μl