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. Author manuscript; available in PMC: 2020 Jan 28.
Published in final edited form as: Analyst. 2019 Jan 28;144(3):753–765. doi: 10.1039/c8an01525b

Figure 3.

Figure 3.

Multiplex coherent anti-Stokes Raman scattering (CARS) imaging of 3T3-L1-derived adipocyte to map the composition and packing of individual lipid droplets. Cells were incubated in a 1:3 mix of unsaturated : saturated fatty acid (A) Brightfield image of an adipocyte. Spontaneous Raman-like spectra in the (B) CC-stretch and (C) CH-stretch regions for locations indicated (in D). Retrieved spectra was then analyzed for mapping the (D) lipid concentration, (E) acyl chain unsaturation and (F) acyl chain order on the same adipocyte. Reprinted from Biophysical Journal, Volume 95, Issue 10, H. A. Rinia, K. N. J. Burger, M. Bonn and M. Müller, Quantitative Label-Free Imaging of Lipid Composition and Packing of Individual Cellular Lipid Droplets Using Multiplex CARS Microscopy, Pages 4908-4914, Copyright (2018), with permission from Elsevier.