Figure 6.
Stimulated Raman scattering (SRS) image processing pipeline for determining cellular boundaries and characterizing lipid droplets in single cells. (A) three-dimensional lipid-specific images were acquired at 2850 cm-1. The signal was processed to generate a lipid droplet mask. The lipid droplet mask was analyzed for three-dimensional morphological characterization (B) three-dimensional protein-specific images were acquired at 2950 cm-1 for cell boundary segmentation and cell mask generation. The position of each LD was then recorded and assigned to an individual cell. Reprinted with permission from C. Cao, D. Zhou, T. Chen, A. M. Streets and Y. Huang, Anal. Chem., 2016, 88, 4931–4939 Copyright (2018) American Chemical Society.
