Figure 7.

Combining lipidomic and genomic analysis at the single-cell level. (A)Lipidomic and genomic analysis using microfluidic single-cell isolation. A single cell is physically isolated in a small chamber using valve-based compartmentalization. While the cell is trapped, images are acquired in a non-destructive fashion using coherent Raman scattering (CRS) imaging for lipidomic analysis. The cell is then pushed downstream for library preparation and finally sequenced using next generation sequencing (NGS) techniques. (B) Lipidomic and genomic analysis using microscopy and computational cell-segmentation. Multiple live cells are imaged on a coverglass using CRS. Individual cells are then computationally isolated using object recognition algorithms and images are analyzed for lipidomic analysis at the single-cell level. The transcriptome of the same cells is then profiled using in-situ sequencing techniques.