Skip to main content
. 2019 Feb 7;73(3):562–573.e3. doi: 10.1016/j.molcel.2018.11.025

Figure 1.

Figure 1

Cdc45 Is Required for Rad53-Dependent Phosphorylation of Sld3 In Vivo

(A) Western blots (left) and flow cytometry (right) of the indicated yeast strains released from G1 arrest in alpha factor (time 0) into 200 mM HU. sml1Δ is required for the viability of rad53Δ strains.

(B) As in (A), except strains were arrested in nocodazole and treated with 10 μg/mL 4-NQO.

(C) As in (A), except that Mrc1–13myc was resolved on a PhosTag gel.

(D) As in (B), except strains were first arrested at 25°C and then shifted to 37°C before addition of 4-NQO.