Figure 5.
Cdc45 Targets Rad53 to Replication Forks
(A) Anti-HA ChIP of the indicated strains released from alpha factor (G1) into 200 mM HU for 60 min. Data are presented as a heatmap of sequencing reads normalized to the reads from 500 bp on either side of all yeast centromeres. The map is centered on 322 of the yeast origins, arranged by increasing trep.
(B) Data from (A) were normalized to the reads from Cse4-HA-tagged strain alone. Graph represents an average signal from 322 origins, centered on the origin.
(C) Rad53 western blot from indicated strains as in Figure 1A.
(D) Immunoprecipitation of Mcm4-HA in the presence of the crosslinking agent EGS from the indicated strains released from alpha factor (G1) into 200 mM HU for 60 min.
(E) As in (D), except performed in the presence of DSP, not EGS. The sld3-A allele is Myc-tagged.