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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Anesthesiology. 2019 Mar;130(3):423–434. doi: 10.1097/ALN.0000000000002528

Figure 2: Confirmation of the specificity of the Cre-lox system by immunohistochemistry of the Ai6 reporter line.

Figure 2:

Confocal images of the 4-hydroxy tamoxifen injected Pgfap-CreERT2/+;ai6/+ and ai6/+ mice brain slices. A. ai6/+ mice which only express a green marker (Zsgreen) when activated by the Cre-recombinase were used as controls. Nuclei were stained with DAPI (blue), astrocytes with anti-GFAP (red) and Zsgreen with anti-GFP (green). There is no green seen in astrocytes since there is no Cre-recombinase acting in these animals. B. Immunohistochemistry using anti-GFAP antibody reveals the co-localization of Zsgreen in the astrocytes of Pgfap-CreERT2/+;ai6/+ reporter mice when Cre-recombinase is activated by 4-hydroxy tamoxifen. Thus, the localization of Zsgreen marks those cells with Pgfap driven Cre-recombinase mediated excision of the floxed gene.